HEK Cell Line Expressing Human EP2 Prostanoid Receptors

Technology #ua04-050

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John Regan
Professor, Pharmacology & Toxicology
Managed By
Tod Mccauley
Title: HEK Cell Lines Expressing Human EP2 Prostanoid Receptors
Basis of Invention: University of Arizona researchers have created human embryonic kidney cells expressing the human EP2 prostanoid receptors. The new cell lines are expected to be a useful research tool for investigating receptor activity: they can be used in functional studies of receptor activation and second messenger signaling. They will also provide a sensitive and reliable measure of the potential for drugs to interact with the receptor.
The Technology: Nucleotides 157-1233 from the human EP2 prostanoid receptor had been previously cloned into the Bluescript-KS(+) plasmid. This plasmid was digested with KpnI and BamH1 restriction enzymes, and the product was cloned into pCEP4, and transfected into 293-EBNA cells. Transformed lines were selected with hygromycin. The resulting protein was selected by PGE2 stimulation of cAMP formation. It was further characterized by immunofluorescence microscopy with a human EP2 receptor antibody and radioligand binding of [3H] prostaglandin E2.
Highlights: *              Stably transformed cell lines *              Useful in research into receptor activity and signaling *              Sensitive and reliable measure of the potential for drugs to interact with the receptors.
Related UA technologies: UA04-052, UA04-053, UA04-081, UA04-051
State of Development. Have stable cell lines expressing the receptors.