Infectious Myonecrosis Virus (Imnv) Nucleotide Sequence, Primers for Rt-Pcr and a DNA Probe for in Situ Hybridization Assays for Detection of the Virus in Shrimp

Technology #ua04-071

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Researchers
Feng-Jyu Tang-Nelson
Associate Research Professor, Veterinary Science & Microbiology
Bonnie Poulos
Assistant Staff Scientist, Ecology & Evolutionary Biology
Donald Lightner
Professor, Veterinary Science & Microbiology
Managed By
Tod McCauley
Sr. Licensing Manager (520) 626-7916

Background: Infectious myonecrosis virus (IMNV) infecting cultured Litopenaeus vannamei, identified first  in Brazil, is a double-stranded RNA virus that causes a slowly progressive disease with cumulative mortalities of up to 70%. The disease has spread to Indonesia and other shrimp aquaculture sites. The disease is commonly diagnosed using a combination of gross signs (primarily skeletal tail muscle necrosis with white opaque discoloration), histopathology, and in situ hybridization with a digoxigenin-labeled gene probe.

Technology: University researchers have developed a rapid and sensitive method for definitive diagnosis of the disease using reverse-transcriptase polymerase chain reaction (RT-PCR). Two primer sets were used to detect 328 and 139 bp amplicons in a nested RT-PCR assay.

Advantage:

The primers were shown to be specific for IMNV and no amplicons were detected using RNA extracted from shrimp infected with other penaeid shrimp viruses (Taura syndrome virus (TSV0, yellowhead virus (YHV), infectious hypodermal hematopoietic necrosis virus (IHHNV) and white spot syndrome virus (WSSV).

Lead Investigator: Donald V. Lightner, PhD

Status: Available for non-exclusive licensing

Refer to Case # UA04-071