A Device and Methods of the Use of Coated Particles for the Purification, Concentration, Management, and Storage of BiomoleculesTechnology #ua05-015
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Title: A Device and Methods of Use of Coated Particles for the Purification, Concentration, Management and Storage of Biomolecules
The Technology: Researchers at the University of Arizona have developed a series of methods for the purification, concentration, management, and storage of biomolecules. This will allow for the analysis of molecules such as DNA or RNA, immunoglobin proteins and specific proteins from a variety of complex sample sources. This simple technology can be performed without the requirement for expensive machinery, at a reduced cost, and without the limitations on sample size commonly associated with the current industry standards that focus on column chromatography.
Background: The progression of scientific research relies upon the availability of commercial equipment utilized to acquire data. The general market has been swamped with affinity matrix based column chromatography processes used to collect biomolecules such as RNA and DNA for nearly 40 years. However, to practice these techniques requires access to a standard wet laboratory equipped with highly expensive lab equipment such as high speed centrifuges and utilizes a great deal of costly consumable plasticware. A new standard must be adopted that enables researchers to conduct the same types of biomolecular collections without such a dramatic increase in laboratory costs.
- Purification of DNA and RNA from complex samples such as blood, buccal washes and environmental fluids
- Development of devices to purify and concentrate immunoglobin proteins from blood, serum, and cell culture filtrates
Concentration and purification of specific proteins from complex mixtures
- More flexible range for sample batch size processing allowing for analysis upon samples greater than the standard 1-5mL range
- Analysis can be performed independent of hardware associated with column chromatography such as high speed centrifuges
- Simple process to perform using conventional laboratory tools as a low cost of manufacture and packaging
Pelleting of DNA samples allows for potential storage upon a benchtop at ambient room temperatures