Lipid Membranes Enabling Maldi-Tof Mass Spectrometry Protein Analysis

Technology #ua15-210

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Researchers
Steven Scott Saavedra
Professor, Chemistry & Biochemistry
Managed By
Paul Eynott
Sr. Licensing Manager (520) 621-2878

Title: Lipid membranes enabling MALDI-TOF mass spectrometry protein analysis

 

Invention: The technology is the use of planar supported lipid bilayer to create the stability for matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) analysis.  

 

Background: MALDI-TOF is an effective method to detect biological analytes from complex samples. MALDI-TOF MS is effective to extract biological analytes by label-free detection and identification. Unfortunately, these proteins bind to membrane-associated receptors, making it incompatible for MALDI-TOF MS with artificial lipid membranes. Cross-linking polymerization of planar supported lipid bilayer provides the stability for MALDI-TOF MS analysis of transmembrane proteins.

 

Applications:

  • Bio-sensing for developing improved transmembrane protein detection tools
  • Detection tool in novel drug targets and processes

 

Advantages:

  • Proteins bound to membrane-associated receptors make it incompatible for MALDI-TOF MS with artificial lipid membranes
  • Cross-linking polymerization of planar supported lipid bilayer provides the stability for MALDI-TOF MS analysis of transmembrane proteins
  • Technology does not require antibodies or a label unlike current protein array options
  • Minimal possibility of cross-sensitivity

  

Licensing Manager:

John Geikler

JohnG@TLA.arizona.edu

(520)626-4605