Stabilized Vesicle-Functionalized Microparticles for Chemical Separations

Technology #ua16-036

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Categories
Researchers
Craig Aspinwall
Professor, Chemistry
Jinyan Wang
Graduate Assistant, Chemistry & Biochemistry
Kendall Sandy
Graduate Assistant, Chemistry & Biochemistry
Steven Scott Saavedra
Professor, Chemistry & Biochemistry
Managed By
Paul Eynott
Sr. Licensing Manager (520) 621-2878

Title:   Stabilized vesicle-functionalized microparticles for chemical separations

Invention: This invention relates to bioassay platforms, in particular, pull-down assay platforms using silica core-polymerized phospholipid vesicle shell particles for peptide/protein ligand screening.

Background: Many physiological or pathological events involve the molecular recognition and binding between a peptide/protein ligand and a specific target on the cell membrane. Certain ligand-receptor binding events trigger corresponding cellular responses, such as enzyme activity and gene expression. Nowadays, screening of peptide/protein ligands that bind to targets on cell membranes is an important process in drug discovery. There is a need for rapid and highly specific assay platforms for identifying novel ligand receptor interactions while minimizing crosstalk and non-specific binding. To address this need this technology provides a novel microparticle architecture that utilizes stabilized vesicle-functionalized microparticles for separations.

Applications:

  • The stabilized vesicles can subsequently be functionalized with either transmembrane receptors or membrane associated receptors
  • Affinity pull-down assays
  • Other chromatographic separation modalities to provide affinity capture/concentration of low abundance ligands in complex mixtures with minimal sample preparation
  • Electrochemical detection or optical spectroscopy when coupled directly with both electrospray ionization and matrix assisted laser desorption ionization mass spectrometry
  • New families of inexpensive, functional materials for the use in nanomaterial sciences
  • Discovery and identification of unknown ligand/receptor pairs with minimal sample preparation

Advantages:

  • A rapid and highly specific assay platform¬†
  • Identifies novel ligand-receptor interactions
  • Minimizes crosstalk and non-specific binding
  • Relies upon interactions existing in nature rather than complex and time consuming synthesis and selection of antibodies
  • Potential to reduce interference from complicated signaling events in cell-based functional assays
  • Identifies an unknown ligand from a ligand mixture
  • Provides quantitative analysis of the binding complement due to the stability of the polymeric phospholipid vesicles

Licensing Manager:

Paul Eynott

PaulE@tla.arizona.edu

(520) 471-2687