Method for 3D Nonlinear Structured Illumination Super-Resolution Imaging

Technology #ua16-143

Questions about this technology? Ask a Technology Manager

Download Printable PDF

Han Zhang
Graduate associate, Optical Science
Yu Li
Postdoctoral Associate, Optical Sciences
Leilei Peng
Assistant Professor, Optical Sciences
Managed By
Amy Phillips
Sr. Licensing Manager (520) 621-9579



Title: Use of Low-Coherence Source to Achieve 3D Super-Resolution Imaging 


Invention: Researchers at the University of Arizona have developed a super-resolution microscopy method that is faster, easier to use, and has less artifacts than current super-resolution methods.  The result is a 3D dual-color stimulated emission depletion (STED) nonlinear structured illumination (NL-SIM) microscope. Using a combination of low coherent light and time-gated detection, the microscope has improved lateral and axial resolution.


Background: Current super-resolution microscopy allows live 3D imaging of cellular processes at tens of nanometer resolution, but lacks super-resolution in the axial dimension. The systems are also complex and carry a high risk of artifacts.  There is a need in the art for greater axial resolution, and a system that is easier to use with high quality results.



*  Commercial (hospital/clinic analysis of biological samples)

*  General research tool for studying processes and mechanisms in cells



*  Improved resolution in axial and lateral dimensions

*  Images sample structures as dim as single molecule and as bright as stained and fixed cells under identical experiment parameters

*  High speed reduces artifacts


Licensing Manager:

Amy Phillips

Refer to case number UA16-143