Method for 3D Nonlinear Structured Illumination Super-Resolution Imaging

Technology #ua16-143

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Researchers
Leilei Peng
Assistant Professor, Optical Sciences
Yu Li
Postdoctoral Associate, Optical Sciences
Han Zhang
Graduate associate, Optical Science
Managed By
Amy Phillips
Sr. Licensing Manager (520) 621-9579

UA16-143

 

Title: Use of Low-Coherence Source to Achieve 3D Super-Resolution Imaging 

 

Invention: Researchers at the University of Arizona have developed a super-resolution microscopy method that is faster, easier to use, and has less artifacts than current super-resolution methods.  The result is a 3D dual-color stimulated emission depletion (STED) nonlinear structured illumination (NL-SIM) microscope. Using a combination of low coherent light and time-gated detection, the microscope has improved lateral and axial resolution.

 

Background: Current super-resolution microscopy allows live 3D imaging of cellular processes at tens of nanometer resolution, but lacks super-resolution in the axial dimension. The systems are also complex and carry a high risk of artifacts.  There is a need in the art for greater axial resolution, and a system that is easier to use with high quality results.

 

Applications:

*  Commercial (hospital/clinic analysis of biological samples)

*  General research tool for studying processes and mechanisms in cells

 

Advantages: 

*  Improved resolution in axial and lateral dimensions

*  Images sample structures as dim as single molecule and as bright as stained and fixed cells under identical experiment parameters

*  High speed reduces artifacts

 

Licensing Manager:

Amy Phillips

AmyP@tla.arizona.edu

Refer to case number UA16-143