Invention:
This invention relates to a platform that couples bead-based immunoassays with 3D traction force microscopy (TFM) within a single hydrogel substrate to quantify time-resolved biochemical cues and cell-generated mechanical forces at matched locations. This invention allows researchers to directly test how local molecular cues influence cell mechanics and behaviors.
Background:
Conventional traction force microscopy (TFM) measures cellular mechanics but does not simultaneously quantify localized biochemical signaling. Current approaches require collecting culture media for separate immunoassays, which disrupts spatial context and prevent direct correlation between force generation and protein secretion. Micropost arrays and planar sensors can localize forces but lack volumetric displacement mapping and integrated biochemical capture. This technology improves on existing methods by enabling in situ, spatially registered immunoassay readouts alongside traction force measurements, preserving native geometry and timing while directly linking mechanical forces to localized biochemical activity.
Applications:
- 3D traction force microscopy platform (TFM)
- Bead-based immunoassay platform
- Mechanobiology
- Oncology
- Immunology
- Drug/biologic screening
Advantages:
- Near-real-time or windowed biochemical readouts
- Measures both biochemical and mechanical cell outputs
- Compatible with standard confocal microscope and widely used hydrogel chemistries
- Amenable to multi-well plates and automated imaging/analysis
